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7952667 
Journal Article 
Stereoselective high-performance liquid chromatographic determination of the enantiomers of ketamine and norketamine in plasma 
Geisslinger, G; Menzel-Soglowek, S; Kamp, HD; Brune, K 
1991 
1 
Journal of Chromatography
ISSN: 0021-9673 
568 
1 
165-176 
English 
1770094 
WOS:A1991FZ16400016 
An enantioselective high-performance liquid chromatographic assay for the quantitation of the enantiomers of ketamine and its major metabolite norketamine in human plasma is described (assay I). The procedure involved extraction of the compounds from alkalized plasma into cyclohexane. Stereoselective separation was achieved with a prepacked alpha 1-acid glycoprotein column without any derivatization procedure. A second assay using a conventional reversed-phase column to determine total (racemic) ketamine and norketamine is also described. Because of interfering plasma peaks (assay II) the cyclohexane solution was reextracted into 1 M hydrochloric acid. The detection wavelength was 215 nm for all substances. The limit of quantification of the method was ca. 40 ng/ml in plasma. The assays were sensitive and reproducible. The method was demonstrated to be sensitive for stereoselective pharmacokinetic studies of ketamine after clinical doses.