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8034318 
Journal Article 
REPRODUCTION AND LIFE HISTORY OF TWO-LILINED SALAMANDERS (EURYCEA CF. AQUATICA) FROM THE UPPER TENNESSEE RIVER VALLLLEY, USA 
Pierson, TW; Miele, A 
2019 
Yes 
Herpetological Conservation and Biology
ISSN: 1931-7603 
14 
1 
111-118 
WOS:000466559700010 
The Two-lined Salamander (Eurycea bislineata) species complex is widely distributed across semiaquatic habitats in various ecoregions of the eastern U.S. and Canada of North America. For these reasons, the complex represents a fruitful model for studying the influence of phylogeny and environment on life-history variation. Here, we detail almost 2 y of monthly life-history and demography data from Eurycea cf. aquatica in the upper Tennessee River Valley, a species and region from which such data were previously unreported. Our principle discoveries are a single-year larval period, with hatching in April or May and metamorphosis the following summer, and the occasional presence of males with nests, which have a mean clutch size of 64 eggs. We further comment on male-male aggression and communal nesting, and we frame our results in the broader ecological and evolutionary context provided by previous studies of the E. bislineata species complex. 
amphibians; larval growth; mate-guarding; metamorphosis; plethodontid