Health & Environmental Research Online (HERO)

Print Feedback Export to File
Journal Article 
A novel terpenoid from Elephantopus scaber with antibacterial activity against beta-lactamase-producing clinical isolates 
Jasmine, R; Daisy, P; Selvakumar, BN 
Research Journal of Microbiology
ISSN: 1816-4935 
Academic Journals 
Bioactivity-guided fractionation of the acetone extract of Elephantopus scaber (ES) yielded a new terpenoid, 6-[1-(10,13-dimethyl-4,5,8,9,10,11,12,13, 14,15,16,17-dodecahydro-1H-cyclopenta[α]phenanthren-17-yl)ethyl] -3-methyl-3,6-dihydro-2 H-2-pyranone. The structure of the above mentioned compound was elucidated by interpretation of their spectroscopic data. Biological testing of the compound demonstrated significant antibacterial activity against a few multi drug-resistant ESBL-producing clinical isolates. The crude plant extracts demonstrated zones of inhibition in the range of 5-16 mm against the chosen test bacteria. On the basis of promising activity, acetone extracts were selected to determine their efficacy in terms of Minimal Inhibitory Concentration (MIC), which ranged from 1.6-25 mg mL-1. The acetone extract was subjected to activity-guided fractionation. The most effective fraction had a MIC of 62.5-250 μg mL-1. Phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids. © 2007 Academic Journals Inc. 
Bioactivity; Elephantopus scaber; Fractionation; Terpenoid