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8107138 
Journal Article 
An improved HPLC method of analysis of rofecoxib 
Lalla, JK; Hamrapurkar, PD; Yadav, SP; Vyas, PM 
2004 
Yes 
Indian Journal of Pharmaceutical Sciences
ISSN: 0250-474X
EISSN: 1998-3743 
66 
3 
338-340 
English 
An improved, simple, specific and more sensitive method for quantitative determination of rofecoxib from the human plasma is described. The method consists of pre-column photoactivation of rofecoxib in acetonitrile by exposure to UV light at 366 nm. The drug probably undergoes a stilbene-phenanthrene like photocyclization reaction with resulting formation of a highly fluorescent species. Subsequent injection on C18e column in HPLC with fluorescent detector at 260 nm excitation and emission at 381 nm with a mobile phase consisting of water:acetonitrile (55:45 v/v) could determine rofecoxib quantitatively in the concentration range of 10 to 350 ng/ml using 20 μl of samples. The assay has been validated and has been successfully utilized in clinical studies.