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8115593 
Journal Article 
间接竞争化学发光酶联免疫分析方法检测禽肉中金刚烷胺和氯霉素残留 
Xu, X; Su, X; Tan, S; Zhong, C; Zeng, D; Zhang, Y; Xu, Z; Wang, X; Yang, J 
2021 
Chinese Chamber of Commerce 
42 
4 
305-312 
Chinese 
An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (ic-CLEIA) was developed for the rapid detection of amantadine and chloramphenicol residues in poultry meat based on efficient sample pretreatment. The dilution factors of coating antigen, antibody and horseradish peroxidase (HRP)-labeled secondary antibody, and competition time were optimized. The results showed that the half-maximum inhibition concentration (IC50) and the linear range were 0.33 μg/L and 0.06-1.77 μg/L for amantadine, and 0.039 μg/L and 0.010-0.179 μg/L for CAP, respectively. Samples were sequentially extracted with ethyl acetate and potassium carbonate solution, and then the extract was blown to dryness with nitrogen gas and purified by n-hexane extraction before being analyzed. The recoveries of amantadine and chloramphenicol from spiked samples were in the ranges of 92.55%-105.14% and 92.00%-112.50%, respectively, and the coefficients of variation were both less than 10.48%. Furthermore, the results determined by ic-CLEIA were highly correlated with those of instrumental analysis method (R2 > 0.98), which demonstrates that the established ic-CLEIA method has high accuracy and good reliability. This method represents a rapid immunoassay for the detection of veterinary drug residues in poultry meat. © 2021, China Food Publishing Company. All right reserved. 
Amantadine; Chloramphenicol; Indirect competitive chemiluminescence enzyme-linked immunosorbent assay; Poultry meat; Pretreatment