US EPA

8150585 

Journal Article 

Isolation of alkaloids from plant materials by the combination of ion-pair extraction and preparative ion-pair HPLC using sodium perchlorate. I. Magnoliae Cortex 

Moriyasu, M; Ichimaru, M; Nishiyama, Y; Kato, A 

1994 

Yes 

Journal of Natural Medicines
ISSN: 1340-3443
EISSN: 1861-0293 

48 

4 

282-286 

English 

Quaternary alkaloids in Magnoliae Cortex were isolated by the ion-pair extraction-preparative ion-pair HPLC method, in which sodium perchlorate was utilized as the ion-pair reagent. Quaternary alkaloids form ion-pairs with perchlorate anion and the resulting ion-pairs are extractable with 1,2-dichloroethane under acidic conditions. The quaternary alkaloids thus obtained were efficiently separated by ion-pair HPLC in which sodium perchlorate was also utilized. When preparative HPLC was used, the effluent was concentrated to remove the organic solvent such as acetonitrile, and by extracting the residue with 1,2-dichloroethane and evaporating 1,2-dichloroethane, quaternary alkaloids were obtained as perchlorate of pure alkaloids. Seven quaternary bases were thus isolated from Magnoliae Cortex and their structures were elucidated. Of them, (+)-laurifoline, (±)-oblongine, (+)-menisperine, (+)-xanthoplanine and (+)-N-methylglaucine were isolated for the first time from Magnolia obovata. 

ion-pair extraction; ion-pair HPLC; Magnolia obovata; quaternary alkaloids; sodium perchlorate