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8170695 
Journal Article 
Industrial application of xylanase in the crude enzyme extract from Trichoderma sp.MS 2010 
Sathiyavathi, M; Parvatham, R 
2013 
6 
SUPPL. 2 
90-94 
English 
In this study, Trichoderma sp.MS 2010 (GenBank accession number, HM 124450) was used as the source for xylanase. Sugarcane bagasse, an inexpensive agro-industrial waste rich in lignocellulosic materials exhibited maximum production of xylanase. It was found that initial pH 5.0, cultivation temperature of 27°C, incubation period of 10-12 days and 80% moisture content as the optimum growth conditions required for the highest production of xylanase by Trichoderma sp MS 2010. Among the nitrogen supplements, both organic and inorganic nitrogen sources increased the production of xylanase. Inorganic metal salts like ZnSO4, MnSO4, MgSO4, FeSO4, NaCl, and CaCl2 were found to support xylanase production. Prebleaching of pulp by xylanase in the crude enzyme extract followed by multistage chemical treatment resulted in enhancement of the brightness, which was attained with 50% reduction in chemical consumption. 
Prebleaching; Sugarcane bagasse; Trichoderma sp MS 2010; Xylanase