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8172491 
Journal Article 
Antimicrobial and antioxidant activities of crude methanol extract and fractions of Andrographis paniculata leaf (Family: Acanthaceae) (Burm. f.) wall. Ex Nees 
Banji, A; Goodluck, B; Oluchi, O; Stephen, F 
2018 
Hashemite University 
11 
1 
23-30 
English 
Assessment of the antimicrobial and antioxidant activities of different solvent fractions of crude methanol extract of Andrographis paniculata leaf was carried out to investigate their medicinal properties. The test samples, crude methanol eextractt and its n-hexane, ethyl acetate, chloroform and water soluble fractions were tested against five clinical isolates: Enterobacter cloacae, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Candida albicans. All the test samples showed antimicrobial activity against the test organisms, except for Escherichia coli which showed resistance to all the samples at the studied concentrations and Candida albicans which showed resistance to all the samples except for ethyl acetate with diameter zones of inhibition ranging from 11.5-17.5 mm and water soluble fractions with diameter zones of inhibition ranging from 11.5-13.0 mm; both in concentration dependent manner. The highest flavonoid content (41.79±0.44 μg QE/mg) and phenolic content (26.79±0.66 μg GAE/mg) were shown by the crude methanol extract and the n-hexane fraction, respectively while the chloroform fraction showed the least concentrations for both flavonoid (11.66±0.12 μg QE/mg) and phenolic (16.17±0.61 μg GAE/mg). In vitro antioxidant study using 2, 2-diphenyl-1-picrylhydrazyl scavenging assay showed that the crude methanol extract had the highest free radical scavenging activity with average percentage inhibition of 54.50±0.10 while the hexane fraction showed the least with average percentage inhibition of 11.36±0.10. Conclusively, the various solvent fractions of crude methanol extract of Andrographis paniculata; especially the ethyl acetate fraction could be considered a remedy for various infections and diseases which are associated with both the test organisms and free radicals. © 2018 Jordan Journal of Biological Sciences. 
Andrographis paniculata; Antimicrobial; Antioxidant; Flavonoid; Fractions; Phenolic