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8172622 
Journal Article 
DNA interaction studies of Cu (II) and Mn (II) complexes containing mixed ligand of 1, 10-phenanthroline and 2-hydroxy-4-methyl-1, 8-naphthyridine 
Sreekanth, B; Krishnamurthy, G; Naik, HB; Vishnuvardhan, TK; Lokesh, MR; Shashikumar, ND 
2011 
0 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
2 
4 
201-213 
English 
The DNA binding ability of the novel complexes [Cu (L 2) 2L 1] (PF 6) 2 [Complex (1)] and [Mn (L 2) 2L1] (PF 6) 2 [Complex (2)] containing bioactive mixed ligand of the type L 1=2-hydroxy-4-methyl-1, 8-naphthyridine and L 2 =1, 10-phenanthroline were synthesized and structurally characterized by elemental analysis, IR and 1H NMR spectral studies. The intrinsic binding constant Kb has been estimated at room temperature. The binding constant of 2.7 × 10 4 M -1 for complex (1) and 4.3 × 10 4 M -1 for complex (2) in 5 mM Tris-HCl/50 mM NaCl buffer at pH 7.2, respectively, as obtained from absorption spectra indicate that the complexes intercalate between the base pairs of the CT-DNA tightly. The oxidative cleavage activity of the complexes (1) and (2) were studied by using gel electrophoresis and the results show that complexes have potent nuclease activity. 
Cleavage studies; Cu (II) and Mn (II) Complexes; Dna binding