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8176162 
Journal Article 
Phytochelatins and metallothionein-like proteins in Solanum nigrum L. and Conyza canadensis L. roots and leaves under Mn stress 
Wu, H; Gong, C; Liu, P; Wang, Z; Chen, J 
2010 
Yes 
Huanjing Kexue Xuebao / Acta Scientiae Circumstantiae
ISSN: 0253-2468 
30 
10 
2058-2064 
Chinese 
Phytochelatins (PCs) and Metallothionein-like proteins (MTLP) have been suggested as two potential biomarkers for evaluating metal phytoxicity. However, no research has reported that these two compounds exist in plants under Mn stress. This study aimed to investigate the correlations between Mn toxicity and production of PCs and MTLPs in Solanum nigrum L. and Conyza canadensis L. after 30 days exposure to Mn2+ (0.005, 2, 4, 8, 16 mmol · L-1) in hydroponic culture. Plant height and root length of both plants were first increased compared with control(0.005 mmol · L-1) and then decreased gradually. Low contents of PCs were detected in the roots and leaves of the plants under Mn stress. The contents of Glutathione (GSH) and MTLP were more sensitive to Mn stress, and they presented similar trends. MTLP and GSH increased rapidly first and then dropped rapidly with the increase of Mn concentration, indicating that there were correlations between the physiochemical values and Mn concentrations in plant tissues due to the increasing supply of Mn2+. The contents of total non-protein SH compounds(TNP-SH) and GSH were significantly increased (p < 0.05) in 8 mmol · L-1 Mn2+ when compared to the control. The content of MTLP in S. nigrum significantly increased under 8 mmol · L-1 Mn2+, while that in C. canadensis was promoted significantly under 2 and 4 mmol · L-1 Mn2+. The MTLP concentration was maintained at a higher level in S. nigrum in comparison with C. canadensis at the same Mn2+ concentration. GSH and MTLP could be effective response indexes to Mn stress and identification of manganese contaminated soils. S. nigrum showed a stronger tolerance to Mn toxicity than C. canadensis, so it is more suitable for phytoremediation of Mn polluted areas. 
Conyza canadensis L.; Metallothionein-like proteins (MTLP); Mn stress; Phytochelatins (PCs); Solanum nigrum L.