US EPA

8181814 

Journal Article 

HPLC-ELSD同时测定何首乌中6种磷脂类成分的含量 

Cai, ZQ; Luo, YY; Liu, XH; Liu, JX; Sha, XX; Ji, T 

2018 

Chinese Journal of New Drugs Co. Ltd. 

27 

12 

1417-1422 

Chinese 

Objective: To establish a high performance liquid chromatography evaporative light scattering detection (HPLC-ELSD) method for simultaneous determination of PC, PI, PE, PS, PG and LPC in Polygoni Multiflori Radix. from different origins. Methods: The analysis was carried out on a UF-Silica-F52546 silica column (250 mm×4.6 mm, 5 μm) eluted by a mobile phase consisting of N-hexane (containing 0.04% triethylamine, A), isopropanol(B) and 13% acetic acid(C) at a flow rate of 1.0 mL•min-1 at a temperature of 30 ℃. The carrier gas flow rate and drift tube temperature of evaporative light cattering detection were set at 1.5 L•min-1 and 60 ℃, respectively. The result of TOPSIS analysis showed that the comprehensive quality of Polygoni Multifori Caulis sample from Guangzhou was better. Results: The calibration curves of six phospholipids in Polygoni Multiflori Radix. showed good linearity (r≥0.992 3). The average recoveries of the method were between 97.80%~102.02%. Conclusion: The established method is reliable and accurate, and can be used for the quality control of Polygoni Multiflori Radix. © 2018, Chinese Journal of New Drugs Co. Ltd. All right reserved. 

HPLC-ELSD; Phospholipids; Polygoni Multiflori Radix.; Simultaneous determination; TOPSIS