US EPA

8199253 

Journal Article 

Variability of the antibacterial activity of Citrus Limon, Citrus Limetta and Citrus Aurantifolia depending on solvents, extraction methods and conventional growth of multi-resistant strains 

Amal, S; Seloua, K; Ouafae, EY; Bahia, B; Lella, OA; Aicha, LL; Rachid, B 

2021 

1 

Research Journal of Chemistry and Environment
ISSN: 0972-0626 

World Research Association 

25 

2 

7-19 

English 

Our study contributes to the valorization of the three Moroccan species (Citrus Limon, Citrus Limetta and Citrus Aurantifolia) by characterizing them by an in vitro evaluation of the antibacterial activity. We used two extraction techniques: Maceration and Soxhlet. Four different polarity extraction solvents were used (hexane, dichloromethane, ethyl acetate and methanol) to obtain different crude extracts. However, the evaluation of this antibacterial activity of extracts and antibiotics against multi-resistant bacteria is carried out by the disk diffusion method and the macrodilution. We found that methanolic and ethyl acetate Soxhlet extracts of C.Limon, C.Limetta and C. Aurantifolia fruit showed strong antibacterial activity against Staphylococcus aureus and Enterococcus faecalis with minimal inhibition concentrations (MIC) of between 15,62-125 μg/ml and minimum bactericidal concentration (MBC) values of 62,5 to 500 μg/ml. On the other hand, the dichloromethane extracts of the three species proved to be inactive with respect to all the microbial strains tested. Gram + strains proved to be more resistant to antibiotics than gram-strains. This study showed that the solvent, the extraction method and the species significantly affect the yields and antibacterial activity of C.Limon, C.Limetta and C.Aurantifolia fruits. © 2021 World Research Association. All rights reserved. 

Citrus aurantifolia; Citrus limetta; Citrus limon; Extraction method; Solvent and antimicrobial potency