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8202322 
Journal Article 
Evaluation of antidiabetic potential of extract of sonneratia caseolaris (L.) engl. leaves against alloxan-induced diabetes in mice 
Barman, AK; Ahmed, T; Das, H; Biswas, B; Ali, MS; Acharyya, RN; Sarkar, KK; Dev, S 
2021 
Faculty of Pharmacy, University of Benin 
5 
1 
77-83 
English 
Sonneratia caseolaris (L.), a mangrove plant of the Sundarbans have traditionally been used as an antidiabetic agent. The study investigated the potential antioxidant and antidiabetic activities of the ethanol extract of the plant leaves. The antioxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power assay. The total secondary metabolites of the plant such as the total phenolic content (TPC), total tannin content (TTC) and total flavonoid content (TFC) were also evaluated. The antidiabetic potential was evaluated using the in vitro alpha-amylase enzyme inhibitory assay and the in vivo oral glucose tolerance test (OGTT). Furthermore, Swiss albino mice were used to evaluate the effect of extract on blood glucose level of alloxan-induced diabetic mice. The extract showed the presence of a significant amount of phenolics (219.53 mg GAE/g), and flavonoids (454.88 mg QE/g). In the DPPH assay, the IC50 value of S. caseolaris was 27.0 µg/mL, while that of ascorbic acid was 12.0 µg/mL. Reducing power assay also revealed the antioxidative potential of the extract with an RC50 value of 395.5 µg/mL. The extract showed notable alpha-amylase inhibitory activity with an IC50 value of 37.6 µg/mL. In the OGTT test, extract showed a significant (p<0.05) reduction of blood glucose levels compared to control. Moreover, extract of S. caseolaris inhibited the diabetogenic activity of alloxan and significantly (p<0.05) controlled the blood glucose level in treated mice. The result indicated the antidiabetic potential of ethanol extract of S. caseolaris leaves, and therefore, justifies their use in folklore medicine. © 2021 Barman et al. 
Alpha amylase; Antidiabetic; Antioxidant; Sonneratia caseolaris