Purification and reconstitution of the cytochrome P-450 monooxygenase system from uninduced mouse liver | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

8205763

Reference Type

Journal Article

Title

Purification and reconstitution of the cytochrome P-450 monooxygenase system from uninduced mouse liver

Author(s)

Levi, PE; Hodgson, E

Year

1983

Is Peer Reviewed?

Yes

Journal

International Journal of Biochemistry
ISSN: 0020-711X

Volume

Issue

Page Numbers

349-353

Language

English

DOI

10.1016/0020-711X(83)90103-9

Abstract

1. 1. Five fractions containing cytochrome P-450 were purified from uninduced mouse liver by chromatography on phenyl sepharose, DEAE-cellulose and hydroxylapatite. 2. 2. The cytochrome P-450 monooxygenase system was reconstituted by combining purified NADPH cytochrome P-450 reductase, cytochrome P-450 and phospholipid. 3. 3. Substrates investigated were benzphetamine, ethoxyresorufin, benzo(a)pyrene, ethoxycoumarin and biphenyl. Â© 1983.