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8208522 
Journal Article 
Antioxidant, anti-cancer and phytochemical constituents of hexane extract fractions of an indian medicinal plant embelia ribes burm 
Vadivel, V; Abirami, K; Ravichandran, N; Brindha, P 
2016 
0 
International Journal of Pharmacognosy and Phytochemical Research
ISSN: 0975-4873 
International Journal of Pharmacognosy and Phytochemical Research 
8 
2 
228-234 
English 
Embelia ribes Burm. (Family: Myrsinaceae, Vidangam in Tamil) has been used as herbal drug for various diseases in Indian medicinal systems. Many research works have been focused on embelin (a high polar bioactive constituent), no study found on low polar compounds. Hence, in the present work we have analyzed the antioxidant and anticancer properties of three different fractions obtained from hexane extract of E. ribes fruit and also to reveal the volatile phytochemicals profile. Three fractions were obtained from hexane extract of Embelia ribes Burm using hexane-chloroform (80:20, V/V) in column chromatography. Fraction I (orange red colour), fraction II (brown colour) and fraction III (dark green colour) were analyzed for antioxidant (DPPH assay) and anticancer activities (EAC cell line model) and also the phytochemical profile was investigated using GC-MS. Among the three fractions investigated, fraction II exhibited higher level of antioxidant effect in terms of phosphomolybdate reducing power (IC50 0.21 mg/ml), DPPH radical scavenging activity (IC50 1.04 mg/ml) and superoxide radical inhibition activity (1.58 mg/ml). The anticancer assay also revealed that the fraction II was effective to control the growth of EAC cell lines (IC50 value 4.36 mg/ml) when compared to other fractions. GC-MS analysis revealed the presence of major volatile phytochemical constituents such as Dodecanoic acid and Ethyl tridecanoate, which could be responsible for the medicinal effects exhibited by the fraction II. Thus, fraction II of hexane extract of Embelia ribes with remarkable antioxidant and anticancer activities could be considered for further evaluation as anticancer drug in animal models. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. 
Column fractions; DPPH assay; EAC cell line; GC-MS analysis; Vidamngam