Objective: To investigate the elemental contents of methanolic soxhlet leaf extract of Ampelocissus latifolia (Roxb.) Planch. by ICP-AES technique. The quantitative estimation of various concentrations of elements in medicinal plants is necessary for determining their effectiveness in treating various diseases and for understanding their pharmacological action. Methods: Elemental concentrations of methanolic soxhlet leaf extracts of Ampelocissus latifolia was measured by the ICP-AES technique. 41 elements Na, Mg, Si, Cl, K, Ca, Cr, Mn, Fe, Ni, Cu, Zn, Co, Cd, Se, Al, S, Pb, Ba, Hg, As, B, P, Sr, Br, Ti, Bi, Ge, In, La, Li, Mo, Pd, Sb, Sc, Sn, Te, V, W, I, Th were screened. Results: Depending on geo-environmental factors and local soil characteristics, 11 elements were found to be present in the above leaf extract in different concentrations. 41 elements and their role in treating various diseases are discussed in this research paper. Conclusion: Data on elemental concentration in the above medicinal plant will be useful to set new standards for prescribing the dosage and duration of administration of these herbal medicines.