US EPA

8211158 

Journal Article 

Production of lignin degrading enzymes namely Laccase, Manganese Peroxidase (MnP) and Lignin Peroxidase (LiP) of selected white-rot fungus in solid state fermentation (solid media) 

Parikh, J; Nadagouda, V; Shukla, MD 

2014 

1 

Research Journal of Biotechnology
ISSN: 0973-6263 

9 

10 

80-82 

English 

The production of the lignin degrading enzymes namely Laccase, Manganese Peroxidase (MnP) and Lignin Peroxidase (LiP) of selected white-rot fungus was examined in this study. Two fungal cultures were studied, both belonging to Ganoderma sp. After screening the cultures for the presence of the enzymes Laccase by Plate Assay techniques, quantitative analysis of all the three enzymes was done by Spectrophotometric method. The effect of different carbon sources namely ligninocellulose containing Rice bran and Wheat bran was examined on the production of these enzymes in solid media. Both the cultures showed diverse enzyme production of all the three enzymes in Rice bran and Wheat bran media. This study demonstrated that media manipulation can change the enzyme production in the fungal cultures. By optimizing the media condition we can maximize the desired enzyme production. 

Ganoderma sp.; Laccase; Lignin Peroxidase (LiP); Ligninocellulose; Manganese Peroxidase (MnP)