US EPA

8216668 

Journal Article 

Inhibitory activity of terpenoid from the medicinal plant Andrographis paniculata against biofouling bacteria 

Sarala, V; Radhakrishnan, M; Balagurunathan, R 

2011 

0 

International Journal of ChemTech Research
ISSN: 0974-4290 

3 

3 

1225-1231 

English 

Biofouling bacteria were isolated from fouling sample collected from boat and other marine structures around Parangipettai coastal area (lat.11° 29′N; long.79° 46′E) and its population was estimated as 8.15×106 CFU/gram. Based on the result of adherence study, five different biofouling bacterial isolates were selected, characterized and identified as Pseudomonas sp., Alteromonas sp., Bacillus sp., Staphylococcus sp., and Serratia sp. Crude bioactive metabolites from 15 plant samples (leaves) were collected and were extracted using methanol and tested against biofouling bacteria by disc diffusion method in which nine extracts showed inhibition against biofouling bacteria. Andrographis paniculata was selected for further isolation of antifouling compound, as it showed maximum of 13-21mm zone of inhibition. Of the various solvents tested for extraction methanol extract showed best activity followed by ethanol, ethyl acetate, n-hexane and aqueous extract. Antifouling compound was separated by TLC. The active compound separated in TLC was detected by bioautography in which the first spot (Rf value - 0.96) showed antifouling activity. Based on the results of phytochemical analysis the active compound was identified as terpenoids. The partially purified fraction was tested for biofouling inhibition and prevention by cover glass method, and wooden stick method. In both the studies the partially purified fraction showed good activity. Further investigation of active compound from A. paniculata will leads to the development of economically cheaper and ecofriendly antifouling compounds. 

Andragraphis paniculata; Antifouling compounds; Biofouling; Terpenoids