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8218866 
Journal Article 
In vitro evaluation of xanthine oxidase inhibitory activity of Sonchus arvensis leaves 
Hendriani, R; Sukandar, EY; Kusnandaranggadiredja; Sukrasno 
2014 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
IJPPS 
6 
2 
501-503 
English 
Objective: This studyexaminedthe inhibiting effects of different extracts of S.arvensis leaves onthe activity of xanthine oxidase, an essential enzyme for uric acid synthesis. Methods: Activity test was conducted in vitro by measuring the activity of xanthine oxidase using UV spectrophotometry. Results: The IC50 of ethanolic extract of S. arvensis leaves was 23.64 μg/mL. Meanwhile, the n-hexane, ethylacetateandwater fraction of the extract had respective IC50 of 263.19, 16.20 and 141.80 μg/mL. When the leaves were extracted with ethyl acetate, the extract thus obtained showed an IC50of 15.29 μg/mL.Allopurinol, as reference drug, had IC50 of 4.84 μg/mL. Conclusion: Ethyl acetate extract of S. arvensis leaves potential to be developed as agent to treat hyperuricemia and gout. 
Gout; In vitro; Inhibitory effect; Sonchusarvensis; Xanthine oxidase