Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
8218876
Reference Type
Journal Article
Title
Separation and analysis of seven kind compounds in three medicine herbs extraction by RP-HPLC
Author(s)
Lee, KJ; Lee, TKKJ; Gu, MJ; Lee, JH; Kim, HK; Ma, JY
Year
2014
Is Peer Reviewed?
Yes
Journal
Asian Journal of Chemistry
ISSN:
0970-7077
EISSN:
0975-427X
Publisher
Chemical Publishing Co.
Volume
26
Issue
22
Page Numbers
7811-7816
Language
English
DOI
10.14233/ajchem.2014.17911
Abstract
Saururus chinensis, Polygalae radix and Curcuma longa are widely known as traditional oriental medicinal herbs with various pharmacological activities. These three herbs were efficiently identified with 60% aqueous ethanol and then successively partitioned with hexane, dichloromethane, ethyl acetate, normal butyl alcohol and water to investigate the seven compounds rutin, quercitrin, quercetin, onjisaponin B, bisdemethoxycurcumin, demethoxycurcumin and curcumin. The samples of these medicine herbs were simultaneously separated and analyzed by commercial C18 reverse phase high-performance liquid chromatography using the combination of water/ trifluoroacetic acid and acetonitrile as the mobile phase in the isocratic and gradient elution mode. The analysis conditions were as follows: flow rate 1 mL/min, injection volume 10 μL and column oven temperature 40 ° C at 200-400 nm wavelengths. The yield (%) and total extraction amount (mg) of n-BuOH (11.88-18.80%, 1.2-9.4 mg) were higher than those of the other partition solvents hexane, dichloromethane and ethyl acetate. Also, the optimum mobile phase was simultaneously separated and analyzed. These results suggest that the extracts of these three medicinal herbs can be good therapeutic materials for anti-oxidant and anti-angiogenesis activities in the treatment of obesity.
Keywords
Analysis; HPLC; Optimization; Purification; Separation
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity