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8220032 
Journal Article 
Fatty acid composition and antidermatophytic and antidiarrheal activity of nelumbo nucifera seed oil 
Arumugam, A; Dhailappan, A 
2012 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
4 
SUPPL. 4 
264-270 
English 
The seeds of Nelumbo nucifera were collected from different localities of Coimbatore District. Extraction of oil from plant seed was performed using Soxhlet's procedure. Based on the physicochemical screening the lotus seed was found to be an excellent source of crude oil. The crude fat content of the ground seeds of N. nucifera was determined by GC-MS and identified by spectral library comparison and by the mass spectral fragmentation pattern. HPTLC analysis showed the presence of flavonoids. The hexane extract of the N. nucifera seed oil showed 66% inhibition of the radical DPPH. Whereas the standard ascorbic acid showed IC50 value of 15 ± 1.0 μg/ml. ABTS activity of N. nucifera seed oil (IC50 = 124 ± 1.0 μg/ml) was observed as the strongest activity. The antidiarrhoeal activity of N.nucifera seed oil was evaluated and it strongly inhibited the strains namely Shigella sp., Salmonella sp., Klebsiella sp., Escherichia Coli., Pseudomonas sp., and Staphylococcus aureus by disk diffusion method. The inhibitory activity of N.nucifera seed oil was pronounced against the dermatophytes like Malassezia furfur, Trichophyton rubrum and Trichophyton mentagrophytes with the extract concentration of 25μg/mL. This study systematically supports the usage of seed as a medicine for superficial bacterial and fungal infections, alternative source of nutrition and as well as renewable resources. 
Agar disc diffusion; Antidiarrhoeal and antidermatophytic activity; Antioxidant; GC-MS; HPTLC; Nelumbo nucifera