US EPA

8220108 

Journal Article 

Antioxidant and anticandidal activity studies on phyllanthin compound from Phyllanthus niruri 

Anusha, , JR; Remya, RV; Sasi Premila, JM; Fleming, AT 

2014 

1 

International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 

IJPPS 

6 

SUPPL. 2 

323-326 

English 

Objective: The present study was to investigate the phyllanthin present in Phyllanthus niruri which belongs to the family, Euphorbiaceae. The vital aim was to evaluate the antioxidant and to determine the anticandidal efficacies against Candida albicans. Methods: Phyllanthin, is one of the active lignan which was isolated from Phyllanthus niruri by silica gel column chromatography employing gradient elution with hexane-ethyl acetate solvent mixture. The eluted samples were further identified and estimated using HPTLC. Free radical scavenging activity of phyllanthin was examined using DPPH assay. Anticandidal activity was studied by analysing the growth inhibition and Minimum Fungicidal Concentration (MFC) rate. Results: The retention time of phyllanthin is 23.4 and its total run time was 45 min. Phyllanthin exhibited very high antioxidative property by its low IC50=7.5 μmol/mL. The isolated phyllanthin alters the growth of C. albicans and showed significant results in vitro. Among the various concentrations of phyllanthin, 250 μl/L concentration revealed the high rate of inhibition. Conclusion: The phyllanthin compound from the medicinal plant, Phyllanthus niruri confirmed as an effective antifungal agent to prevent the candidiasis and the secondary infections caused in various diseases. 

Anticandidal activity; Antioxidant; Candida albicans; Phyllanthin; Phyllanthus niruri