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8224234 
Journal Article 
Transesterification of Linoleic acid in grape seed [Vitis vinifera l.]oiland its analytical method development using gas chromatography 
Damayanti, S; Sigi, VA; Julianti, E 
2014 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
IJPPS 
6 
4 
528-531 
English 
Objective: Linoleic acid is an essential fatty acid that humans need to ingest. It is the main compound of grape seed [Vitis vinifera L.] oil which is known as inhibitor of hyperproduction of melanin pigment. The objective of this research is to obtain volatile transesterified linoleic acid and to develop its analytical method. Methods: Sample A was obtained by soxhletation with n-hexane at 60°C and sample B was commercial grape seed oil obtained mechanically by expeller pressure. Linoleic acid methyl ester was obtained by acid pretreatment with sulfuric acid-methanol and base transesterification with sodium hydroxide-methanol. Results: The optimized condition was resulted at an isothermal column temperature at 190°C for 12 minute whereas the injector and detector were kept at each 250°C and 280°C.Linearity was confirmed over a calibration range with regression coefficients [r] of 0.999 and coefficient variance of linear regression [Vx0] of 1.670%. The limit of detection [LOD] and limit of quantitation [LOQ] of this method were determined from the coefficient of variation of a known concentration of reference standards resulting 38.480 ppm and 116.620 ppm, respectively. Precision test revealed a coefficent of variance of 1.910% for the first day, 0.850% for the second day, and 1.980% for the third day. Accuracy revealed a percentage of recovery in a range of 98.510-99.830 %. Conclusion: Linoleic acid in grape seed [Vitis vinifera L.] oil was succesfully transesterified and its analytical method development using gas chromatography was validated. Concentration of linoleic acid in sample A and B were 60.233 and 70.393%, respectively. 
Gas Chromatography; Grape seed oil; Linoleic acid; Transesterification; Validation of Analytical method