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8224753 
Journal Article 
HPTLC method for the determination of lupeol from Calotropis Procera root bark and Nyctanthes arbortristis leaves 
Rupal, C; Vadalia, KR; Gokani, RG; Chavda, KV 
2013 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
5 
2 
547-550 
English 
Objective: Development of a simple, rapid, selective and quantitative HPTLC method for determination of lupeol from root bark of Calotropis procera R. Br. and leaf of Nyctanthes arbortristis Linn. Methods: Standard curve for lupeol was obtained by applying different concentration of standard lupeol solution. Different concentration of standard lupeol along with the hexane extracts of Calotropis procera root bark and Nyctanthes arbortristis. Linn. leaf were applied on TLC aluminium plate precoated with Silica gel60 GF254 and developed using benzene: chloroform (1:1) v/v as a mobile phase. The plate was sprayed (derivatized) with 5% v/v aqueous sulphuric acid reagent followed by heating for 10 min. Detection and quantification were carried out by densitometry at wavelength of 366 nm. Percentage of lupeol in both samples was calculated using standard curve. The developed method was validated in terms of linearity, precision, repeatability and accuracy. Results: Percentage of lupeol found in the root bark of C. procera and leaf of N. arbortristis was 0.165% w/w and 0.503% w/w respectively. A good linearity was obtained for lupeol in the range of 0.1-0.6 μg/spot. The mean recovery was close to 100%, which indicates the accuracy of the method proposed. The method was also found specific and reproducible. The limit of detection and limit of quantification were found to be 12.4 ng/spot and 37.57 ng/spot respectively. Conclusion: The method was found to be simple, precise, accurate, specific, reproducible and can be used for routine quality control of C. procera root bark and N. arbortristis Linn. leaf powder, also for the quantification of lupeol in plant materials. 
Calotropis procera; HPTLC; Lupeol; Nyctanthes arbortristis