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8225553 
Journal Article 
Antioxidant and cytotoxic studies on two known compounds isolated from Hyptis suaveolens leaves 
Deepika Priyadharshini, S; Sujatha, V 
2013 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
5 
4 
283-290 
English 
Objective: In the present study, it is intended to investigate the antioxidant profile of Hyptis suaveolens leaves, through numerous in vitro biochemical assays and to isolate useful constituents. This plant has been extensively researched for its essential oils till date. Methods: Various solvent extracts (n-hexane, ethyl acetate, acetone, methanol) were assessed for total content of phenolic, flavonoid, carbohydrate, protein, in vitro antioxidant assays viz. DPPH, superoxide, hydrogen peroxide- radical scavenging assays, Ferric reducing power activity and inhibition lipid peroxidation (TBARS) assays. Bioassay-guided fractionation on ethyl acetate crude was performed using column chromatography which revealed two known compounds characterized through supportive and evidencing spectral data like 1D & 2D-NMR, DEPT and HR-MS. Results: All the performed in vitro assays have revealed the excellence of ethyl acetate extract in contrast to other fractions. Its SC50/ IC50/ EC50 values calculated have recorded significant impact with reference to the standard. Significantly, the estimations viz. phenolic, flavonoid, carbohydrate and protein seem to be aligned with the in vitro antioxidant assays. Thus from the ethyl acetate crude, two bioactive compounds isolated were identified to be menthol and linalool from the spectral evidences. Cytotoxicity (MTT assay) for the two isolates showed moderate cell viability with respect to its concentration, using Cisplatin as positive control. Conclusion: The significance of Hyptis suaveolens in traditional medicine and the importance of its chemical constituents shall improve its role against free radical production. 
Cytotoxic activity; DPPH radical scavenging ability; Ferric reducing power; Linalool; Menthol; Total phenol content