US EPA

8225688 

Journal Article 

Modulatory effects of Chukrasia tabularis A. Juss. bark on in vitro generated oxidative stress 

Kaur, R; Singh, B; Arora, S 

2013 

1 

International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 

5 

SUPPL.1 

158-167 

English 

Introduction: Chukrasia tabularis A. Juss. (Meliaceae), commonly known as Chickrassy is an important medicinal tree of Indian Peninsula and is used for its antimalarial, antipyretic properties since ancient times. Objectives: The present study was designed to explore the modulatory effect of methanol extract of Chukrasia tabularis bark and its different fractions (hexane, ethyl acetate and butanol) on the radicals generated in different in vitro models. Mathods: The methanol extract was prepared using maceration method and resultant extract was fractionated as per the polarity. The in vitro methods used to study antioxidant potential include DPPH, ABTS, molybdate ion reduction, reducing power, hydroxyl radical scavenging i.e. deoxyribose degradation and DNA nicking, superoxide anion radical scavenging and ferrous ion chelation assays. The total phenol and flavonoid content of these fractions was also determined. Results: It was found that methanol extract and its fractions exhibited strong hydrogen donating and hydroxyl radical scavenging ability in all the assays but did not show metal chelation ability. Conclusion: These findings led to the conclusion that methanol extract and its fractions acted as chain breaking antioxidant rather than acting as preventive antioxidants. It was observed that the antioxidant potential of extract and fractions of C. tabularis bark in different assays was linearly correlated to its phenol and flavonoid content. 

Chickrassy; Flavonoids; Hydrogen donation; Hydroxyl radicals; Phenols