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8225694 
Journal Article 
Phytochemical and pharmacological analysis of pterodon polygalaeflorus extracts 
Pinto, FA; Vigliano, MV; Leal, NRF; Da Silva, GP; Marques, PR; Velozo, LSM; Sabino, KCC; Coelho, MGP 
2013 
3 
56-70 
English 
Fruits from Pterodon species are commercially available at medicinal flora markets, being largely used in folk medicine by its anti-arthritic, analgesic and anti-inflammatory actions. This study compared the antinociceptive and anti-inflammatory potentials of the ethanol (EEPpg), hexane (HEPpg) and dichloromethane (DEPpg) extracts from Pterodon polygalaeflorus Benth. fruits. Inhibition of nitrite production by RAW 264.7 cells, determined with Griess reagent, presented IC50 values of 11.93 μg/ml (EEPpg), 6.02 μg/ml (HEPpg) and 11.86 μg/ml (DEPpg). In the air pouch model, all extracts reduced the total number of leukocytes in the exudate at all tested doses, in relation to control group, the highest inhibition being observed with HEPpg (62.3%). Macroscopic analysis of the pouch tissues showed reduction of inflammatory signs (redness, vasodilation) on treated mice, mainly with HEPpg, which also reduced microscopically the tissue thickening and leukocyte infiltration. The highest antinociceptive potential (100%) in the writhing test was observed with HEPpg. Phytochemical analysis identified 90% of extract's content, composed by sesquiterpene hydrocarbons, oxygenated sesquiterpenes and furan diterpenes. HEPpg was the extract with the highest antiinflammatory and antinociceptive actions and comparison with literature data suggests a synergy of compounds due to higher antinociceptive activity with a lower dose than that observed with isolated vouacapan derivatives. 
Air pouch model; Antinociceptive activity; Nitric oxide production; Phytochemistry; Pterodon polygalaeflorus