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8241955 
Journal Article 
Extraction, purification and anti-radiation activity of persimmon tannin from Diospyros kaki L.f 
Zhou, Z; Huang, Y; Liang, J; Ou, M; Chen, J; Li, G; , 
2016 
Yes 
Journal of Environmental Radioactivity
ISSN: 0265-931X
EISSN: 1879-1700 
Elsevier Ltd 
OXFORD 
162-163 
182-188 
English 
27267156 
WOS:000390183000023 
In this study, persimmon tannin was extracted from Diospyros kaki L.f. using ultrasound-assisted extraction and purified by D101 macroporous resin column chromatography and polysulfone ultrafiltration membrane. The tannin content of the final persimmon tannin extracts was attained to 39.56% calculated as catechin equivalents. Also, the radioprotective effects of persimmon tannin for HEK 293T cells proliferation and apoptosis after Gamma irradiation were investigated by CCK-8, Hoechst 33258 staining, flow cytometry assay and intracellular reactive oxygen species assay (ROS). Persimmon tannin was pre-incubated with HEK 293T cells for 12 h prior to Gamma irradiation. It was found that pretreatment with persimmon tannin increased cell viability and inhibited generation of Gamma-radiation induced ROS in HEK 293T cells exposed to 8 Gy Gamma-radiation. The percentage of apoptotic cells were only 6.7% when the radiation dose was 8 Gy and pretreated with 200 μg/ml of persimmon tannin. All these results indicated that persimmon tannin offered a potent radioprotective effect on cell vitality and cell apoptosis of Gamma-radiation exposure in HEK 293T cells. This study would serve as a pre-clinical evaluation of persimmon tannin for use in people with radiation protection. 
Anti-radiation; Persimmon tannin; Purification; Ultrasound-assisted extraction