US EPA

8243928 

Journal Article 

In vitro evaluation of stem extracts of polycarpeae aurea wight and arn for their total phenolic content, total flavonoid content and antioxidant potency 

Deepak Raj, K; Ranjit, P; Archana, G 

2020 

1 

Research Journal of Chemistry and Environment
ISSN: 0972-0626 

World Research Association 

24 

12 

61-68 

English 

The aim of this study is to investigate the stem extracts of P. aurea Wight and Arn in various organic solvents for their total phenolic, flavonoid contents and also their antioxidant potency. The highest total phenolic content of the stem extracts was recorded in acetone extract (775.6±8.6) to the lowest in hexane extract (196.16±8.9) (mg GAE/gm) and the highest total flavonoid content was recorded in acetone extract (300.01±5.2) to the lowest recorded in hexane extract (104.56±6.3) (mg QT/gm). Antioxidant potency of various stem extracts was evaluated by DPPH (1, 1-diphenyl-2-picryl-hydrazyl), FRAP (Ferric Reducing Ability of Plasma) and Nitric oxide scavenging. IC50 values for DPPH and NO scavenging activity were recorded as 73.29±3.1 and 87.97±2.9 respectively for stem extracts of P. aurea Wight and Arn in various organic solvents namely acetone, methanol, ethyl acetate, chloroform and hexane. These values are the lowest among various extracts evaluated and showed potent antioxidant properties. The greater concentration of total phenolics and total flavonoids in acetone stem extracts directs to their higher antioxidant activities. Results from the current studies recommend that acetone stem extract of P. aurea Wight and Arn is a promising source of natural free radical scavengers. © 2020 World Research Association. All rights reserved. 

Antioxidant activity; DPPH; FRAP; Total phenolic content