In this study, a high performance liquid chromatography (HPLC) method was established for simultaneous determination of 13-hydroxy-9Z, 11E-octadecadienoic acid (13-Z, E-HODE), 13-hydroxy-9E, 11E-octadecadienoic acid(13-E, E-HODE), 9-hydroxy-10Z, 12E-octadecadienoic acid (9-Z, E-HODE) and 9-hydroxy-10E, 12E-octadecadienoic acid(9-E, E-HODE) in meat products. The analytes were extracted from samples with methanol, purified by Sep-Pak C18 column, and then separated using isocratic elution on an Absolute SiO2 column (250 mm à 4. 6 mm, 5 μm) with a mobile phase consisting of n-hexane, isopropanol and acetic acid (98.3:1. 6:0.1, V/V) before being detected at 234 nm using a photo-diode array(PDA). The results indicated that the method displayed a good linearity within the concentration ranges of 0.5-20.0 μg/mL(R2=0.999 4) for 13-Z, E-HODE, and 0.25-10.0 μg/mL (R2=0.999 2) for 13-E, E-HODE, 0.75-12. 5 μg/mL (R2=0.999 2)for 9-Z, E-HODE and 0.5-7.5 μg/mL (R2=0.999 6) for 9-E, E-HODE, respectively. The detection limits (LODs) for 13-Z, E-HODE, 13-E, E-HODE, 9-Z, E-HODE and 9-E, E-HODE were 0.075, 0.035, 0.090 and 0.060 μg/g, respectively, and the limits of quantification (LOQs) were 0.25, 0.12, 0.32 and 0.20 μg/g, respectively. The average recoveries from spiked samples were 89. 03%, 89. 03%, 89. 33% and 87. 93%, respectively. When this method was used to analyze 18 meat products, all the samples contained 13-Z, E-HODE, 13-E, E-HODE, 9-Z, E-HODE and 9-E, E-HODE at levels of 1.73-9.10, 0.56-5.79, 2.37-11. 02 and 0.78-5.82 μg/g, respectively. In conclusion, this method was proved to be fast, accurate and reproducible, and could be used for the simultaneous quantitative analysis of 13-Z, E-HODE, 13-E, E-HODE, 9-Z, E-HODE and 9-E, E-HODE in meat products. © 2021, China Food Publishing Company. All right reserved.