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Journal Article 
Development and validation of a hptlc method for simultaneous determination of furosemide and spironolactone in its tablet formulation 
Kher, G; Ram, V; Kher, M; Joshi, H 
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 
The objective of the current study was to develop a simple, precise and accurate High Perfomance Thin Layer Chromatographic [HPTLC] assay method and validated for determination of furosemide and spironolactone in solid pharmaceutical dosage forms. The mobile phase comprising of ethyl acetate: haxane in the volume ratio of [80: 20, v/v] was employed for the elution. Standard solution was prepared in methanol. The standard concentration was 40 μg ml-1 of furosemide and 100 μg ml-1 of spironolactone. Chromatographic analysis was performed on a HPTLC plates precoated with 0.25 mm layer of chromatographic silica gel mixture [Silica GF254] on aluminum sheets. After development of the chromatographic plate, the detection was carried out using an Ultraviolet scanning densitometer set at a wavelength of 254 nm. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug the concentration range from 0.016-0.064 mg ml-1 for furosemide and 0.040-0.160 mg ml-1 for spironolactone with correlation coefficient 0.9958 and for Spironolactone with correlation coefficient 0.9975. The (relative standard deviation - RSD) values for intraday precision study and interday precision study was < 2.0 % for furosemide and spironolactone. The mean recovery for furosemide was 98.51-98.81 % and 98.20-98.98 % for spironolactone. 
Assay method; Development and Validation; Furosemide; HPTLC method; Spironolactone