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8248478 
Journal Article 
Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS 
Donot, F; Cazals, G; Gunata, Z; Egron, D; Malinge, J; Strub, C; Fontana, A; Schorr-Galindo, S 
2013 
Yes 
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN: 1570-0232
EISSN: 1873-376X 
Elsevier 
AMSTERDAM 
942-943 
98-106 
English 
24239934 
WOS:000328303800014 
A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45. min through HPLC-APCI-MS/MS technique. © 2013 Elsevier B.V. 
APCI-MS/MS; ELSD; HPLC; Microalgae; Neutral lipids; SPE