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HERO ID
8248478
Reference Type
Journal Article
Title
Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS
Author(s)
Donot, F; Cazals, G; Gunata, Z; Egron, D; Malinge, J; Strub, C; Fontana, A; Schorr-Galindo, S
Year
2013
Is Peer Reviewed?
Yes
Journal
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN:
1570-0232
EISSN:
1873-376X
Publisher
Elsevier
Location
AMSTERDAM
Volume
942-943
Page Numbers
98-106
Language
English
PMID
24239934
DOI
10.1016/j.jchromb.2013.10.016
Web of Science Id
WOS:000328303800014
Abstract
A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45. min through HPLC-APCI-MS/MS technique. © 2013 Elsevier B.V.
Keywords
APCI-MS/MS; ELSD; HPLC; Microalgae; Neutral lipids; SPE
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