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8251021 
Journal Article 
Inhibition of the System Luminol-H2O2-Fe(CN)63- Chemiluminescence by the Mn(II) Indirect Determination of Isoniazid in a Pharmaceutical Formulation 
Gregorio Alapont, A; Aurechia Giménez, E; Lahuerta Zamora, L; Martínez Calatayud, J 
1998 
1 
Luminescence
ISSN: 1522-7235
EISSN: 1522-7243 
13 
3 
131-137 
English 
A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate(III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied to determination of the drug in a pharmaceutical formulation. © 1998 John Wiley & Sons, Ltd. 
Chemiluminescence; Flow injection; Isoniazid; Pharmaceutical analysis; Solid-phase reactors