US EPA

8259063 

Journal Article 

三竹種(Gigantochloa atter, Dendrocalamus asper, Gigantochloa verticillata)竹葉萃取物含量 及清除DPPH自由基活性 

Pujiarti, R; Suryani, S; Sunarta, S; Lukmandaru, G; Purba, BAV 

2020 

Taiwan Forestry Research Institute 

35 

1 

1-12 

English 

In this study, we investigated the extractive contents, phenolic content, and DPPH-scavenging activity of leaf extracts of 3 commonly cultivated bamboo species (Gigantochloa atter, Dendrocalamus asper, and G. verticillata) from Tlogoadi Village, Yogyakarta, Indonesia. Extractions were successively done by reflux, using n-hexane, ethyl acetate, methanol, and hot water. The extracts were subjected to the following procedures: phytochemical screening for alkaloids, saponins, and tannins; a colorimetric assay for total phenolic and flavonoid contents; and a DPPH radical-scavenging capacity assay. Results showed that hot water produced the highest yields of G. atter (3.62%) and D. asper (3.16%), while n-hexane produced the highest yield of G. verticillata (2.46%). Phytochemical screening detected saponins in the hot-water extract of G. atter, and tannins were present in the ethanol (70%, v v-1) extract of all 3 bamboo species. Total phenolic and flavonoid contents were highest in the ethyl-acetate extract of D. asper (26.09 mg gallic acid equivalents g-1) and G. verticillata (92.67 mg quercetin equivalents g-1), respectively. The hot water-soluble extract of G. verticillata showed the lowest 50% inhibitory concentration of 662.41 µg ml-1. The present study indicates that the 3 bamboo leaf extracts can potentially be used as natural sources of antioxidants and phytochemicals, especially the polar extract of G. verticillata with its high flavonoid content and mild DPPH-scavenging activity. © 2020 Taiwan Forestry Research Institute. All rights reserved. 

Antioxidant; Flavonoid; Phenolic; Phytochemical screening; Successive extraction