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8263811 
Journal Article 
接种功能内生细菌对蔬菜亚细胞菲积累的削减作用 
Duan, Z; Gao, N; Zhang, G; Wang, J; Ling, W 
2021 
Yes 
Huanjing Kexue Xuebao / Acta Scientiae Circumstantiae
ISSN: 0253-2468 
Science Press 
41 
4 
1529-1537 
Chinese 
In this study, the changes in phenanthrene contents and enzymatic activities (such as polyphenol oxidase (PPO) and peroxidase (POD)) in water spinach and its subcellular fractions were comprehensively investigated by inoculating a phenanthrene-degrading bacterium Diaphorobacter sp. Phe15, using hydroculture pot experiments. Results showed that strain Phe15 could effectively be colonized into water spinach through root immersion, and the cell counts of Phe15 in each part of water spinach were: root surfaces > inner roots > shoots. Inoculation led to a phenanthrene content reduction of 93.69% and 50.26% in roots and shoots, respectively, resulting in a decreased concentration in the subcellular fractions of water spinach. The phenanthrene content decreased the most in cell soluble fractions. Also, the enzymatic activities of POD and PPO in roots, shoots, and subcellular fractions of water spinach increased with the inoculation of Phe15. Notably, the enzymatic activities of POD and PPO negatively correlated with the contents of phenanthrene in water spinach, indicating that the increased activities of these enzymes would lead to a decrease in phenanthrene content in plants. The findings in this study reveals the underlying mechanisms of endophytic PAH degradation in plants, from the perspective of subcellular fractions and enzyme activity. This study will provide the necessary theoretical basis for using bacterial endophytes in reducing the risks while ensuring agricultural crop safety in PAH-contaminated fields. © 2021, Science Press. All right reserved. 
Endophytic bacteria; PAH-degrading enzymes; PAHs; Subcellular fraction; Water spinach