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8264492 
Journal Article 
Cytotoxic effect of corchorus depressus against hepg2 and hle human liver cancer cells 
Kakrani, PH; Kakrani, HN; Raval, M 
2018 
Innovare Academics Sciences Pvt. Ltd 
11 
12 
187-189 
English 
Objective: The present study was designed to examine the cytotoxic effects of methanolic extract of aerial parts of Corchorus depressus and hexane, chloroform, ethyl acetate, and aqueous fractions of the same extract in the human hepatocellular carcinoma (HCC) (HepG2) and invasive hepatocellular carcinoma cell lines (HLE). Methods: Anti-proliferative effects were evaluated using 3-(4,5-dimethythiazol2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assay. Human HCC (HepG2) and invasive hepatocellular carcinoma cell lines (HLE) were treated with different concentrations of methanolic extract (10, 25, 50, 100, 200, 300, 400, and 500 µg/mL) of aerial parts of C. depressus as well as hexane, chloroform, ethyl acetate, and aqueous fractions (200 µg/mL) for 24 and 48 h. The cell viability and the half maximal inhibitory concentration (IC50) were determined. Results: The maximum cytotoxic effect was noticed with a maximum dose of methanolic extract (500 µg/mL) and alkaloidal fraction (200 µg) in this study with an IC50 value of about 200 µg. Conclusion: The set of studies showed that methanolic extract of aerial parts of C. depressus and alkaloidal, chloroform and ethyl acetate fractions fractions was capable of inhibiting cell growth and cell proliferation by inducing cytotoxicity of HepG2 and HLE cells. © 2018, Innovare Academics Sciences Pvt. Ltd. All rights reserved. 
Corchorus depressus; Cytotoxicity; HepG2 and HLE cell line; Liver cancer; MTT; Neutral red uptake assay