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HERO ID
8306894
Reference Type
Journal Article
Title
Potato seed production of Cultivar Kufri Himalini, in vitro
Author(s)
Badoni, A; Chauhan, JS
Year
2010
Volume
1
Issue
1
Page Numbers
7-10
Language
English
Abstract
The nodal cuttings of potato cv. Kufri Himalini was cultured in MS medium consisting three different hormonal combinations of GA3 and NAA (MSH1- 0.25mg/l GA3+ 0.01 mg/l NAA, MSH2- 0.25mg/l GA3+ 0.03 mg/l NAA and MSH3- 0.25mg/l GA3+ 0.04 mg/l NAA) for shoot and root proliferation. After 35-40 days of incubation, shoots in MSH1 (0.25 mg/l GA3 and 0.01 mg/l NAA) reached 8.28 cm with 9.4 nodes and 11.9 cm root length, higher then all the combinations. For tuberization three concentration of BAP (8 mg/l, 10 mg/l and 12 mg/l) were used with MS liquid medium, the plantlets were shifted to tuberization media and data were reported for the number of microtuber on per original shoot, average weight of microtuber and number of eyes in each microtuber. Formation and development of microtubers were least with 8 mg/l and higher in 10 mg/l BAP concentration, while with the increasing concentration, BAP inhibit the average number, weight and eyes number of microtubers.
Keywords
Hormonal combination; Kufri Himalini; Microtuber; Tuberization
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