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8307239 
Journal Article 
Detection of okadaic acid and related esters in mussels during diarrhetic shellfish poisoning (DSP) episodes in Greece using the mouse bioassay, the PP2A inhibition assay and HPLC with fluorimetric detection 
Prassopoulou, E; Katikou, P; Georgantelis, D; Kyritsakis, A 
2009 
1 
Toxicon
ISSN: 0041-0101
EISSN: 1879-3150 
PERGAMON-ELSEVIER SCIENCE LTD 
OXFORD 
53 
2 
214-227 
English 
19046982 
WOS:000263382200004 
An approach involving chemical, functional and biological techniques was taken for the detection and quantification of the marine toxin okadaic acid (OA) in mussels from Thermaikos and Saronikos Gulfs, Greece, during DSP episodes that occurred in 2006-2007. Samples were analyzed using the mouse bioassay, high performance liquid chromatography with fluorimetric detection (HPLC-FLD), using l-bromoacetylpyrene (BAP), as a precolumn derivatisation reagent, and the protein phosphatase 2A inhibition assay (PP2AIA) using a commercially available kit. Okadaic acid (OA) and its polar and non-polar esters were detected and quantified by HPLC-FLD, after hydrolysis of the samples during preparation. The detection limit of the HPLC method for OA was 5.86 μg OA/kg, which permits this method to be used for the regulatory control of these toxins in shellfish. Comparison of the results by all three methods revealed excellent consistency. © 2008 Elsevier Ltd. All rights reserved. 
Diarrhetic shellfish poisoning; Esters of okadaic acid; HPLC; Mussels; Mytillus galloprovincialis Mouse bioassay; Okadaic acid; Protein phosphatase 2A; Saronikos Gulf; Thermaikos Gulf