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8307335 
Journal Article 
White-light-emitting DNA (WED) 
Varghese, R; Wagenknecht, HA; , 
2009 
Yes 
Chemistry: A European Journal
ISSN: 0947-6539
EISSN: 1521-3765 
15 
37 
9307-9310 
English 
19681079 
A study was conducted to show how to use the DNA backbone for the combination of a donor and an acceptor in such a way that a white-light-emitting DNA is formed upon hybridization. DNA1 bearing the blue-green emitter, ethynyl pyrene and DNA2 bearing the red emitter, ethynyl nile red, were synthesized. Both chromophores were conjugated to the 5-position of 2'-deoxyuridine to maintain the canonical dU:A base pairing. In DNA3-6, both chromophores were combined as an energy donor-acceptor couple and placed adjacent to each other in order to obtain an efficient electronic interaction between them. The phosphoramidite of 5-(1-pyrenylethynyl)-2'-deoxyuridine modified with ethynyl nile red was synthesized by palladium catalysed Sonogashira. The reference duplexes DNA1 and DNA2 showed the characteristic optical behavior of the corresponding chromophores. DNA1 exhibited the absorption and fluorescence maxima of pyrene. 
DNA; Fluorescent probes; FRET; Multichromophores; White-light emission