US EPA

8307453 

Journal Article 

Rapid determination of fat-soluble vitamins with microemulsion capillary electrokinetic chromatography 

Yin, CN; Ding, SD; Gong, WJ; Cao, YH 

2008 

Chinese Academy of Sciences 

36 

9 

1177-1181 

Chinese 

A novel system was developed for the rapid determination of fat-soluble vitamin A, D3, and E with microemulsion electrokinetic chromatography (MEEKC). The microemulsion consists of 1.2% (w/w) sodium lauryl sulphate (SDS), 21% (V/V) 1-butanol, 18% (V/V) acetonitrile, 0.8% (w/w) n-hexane, 20 mmol/L borax buffer (pH 8.4). 1-Butanol as the cosurfactant and acetonitrile as the organic solvent modifier in MEEKC running buffer played an important role for separation of three fat-soluble vitamins. The three analytes were baseline separated within 13 min at 25 kV and 25°C . The RSD values (n = 5) of migration time and peak areas of the analytes were less than 2.5% and 4.5% , respectively. Good linear relations between peak areas and concentration of analytes were established in the range of 20-1000 mg/L for vitamin A, 5-1000 mg/L for vitamin D3, and 5-1000 mg/L for vitamin E. the detection limits (S/N = 3) were 12 ,0.72 and 0.29 mg/L, respectively. This method has been successfully applied for the analysis of commercial vitamin E capsules, the determination results were accorded with the declared in the label. 

Fat-soluble vitamins; Microemulsion; Microemulsion electrokinetic chromatography