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8307737 
Journal Article 
Antihyperuricemic activity of the Kepel [Stelechocarpus burahol (Bl.) Hook. F. & Th.] leaves extract and xanthine oxidase inhibitory study 
Purwantiningsih; Hakim, AR; Purwantini, I 
2010 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
2 
2 
123-127 
English 
Kepel or Stelechocarpus burahol (Bl.) Hook. F & Th. is one of the original plants from Indonesia and has been traditionally used for gout by the Indonesian communities. Kepel has synonyms name as Burahol and Turalak, and some studies showed that the extract and isolated flavonoid from Kepel leaves have antihyperuricemic and antioxidant activity. The aim of this research is to evaluate the antihyperuricemic potency of the ethanol and hexane extract of Kepel leaves as compared to allopurinol. The experiment was divided into two parts, invivo and invitro studies. For invivo study, sixty five rats were used in the study and the rats were divided into 13 groups (n=5 per group). The groups were assigned as follows and the test substances were given orally. Group 1: rats were given 10 ml/kg b.w. of 0.5% CMC-Na; Groups 2-5: rats were given 4.5 to 36 mg/kg b.w. of allopurinol; Groups 6-9: rats were given ethanol extract of Kepel leaves (50 to 400 mg/kg b.w.); and Groups 10-16: rats were given hexane extract of Kepel leaves (50 to 400 mg/kg b.w.). The blood of the test animals were taken through the eye vein on days 15, 17 and 19 post-treatment. Serum uric acid levels was determined by using TBHBA [2,4,6tribromo 3hydroxy benzoic acid] method and the percentage of the degradation was calculated. Inhibitory potency of the ethanol extract to the xanthine oxidase, was done by using procedure Fried & Fried (1993) and compared to allopurinol. Based on this study, it could be concluded that the ethanol and hexane leave extracts of Kepel possess significant antihyperuricemic potency in rat. The antihyperuricemic activity of the ethanol (60.86 to 78.33%) and hexane extracts (78.23 to 88.52%) are almost equivalent to that of the allopurinol (50.82 to 91.16%). While invitro study showed that the ethanol extract has the inhibitory activity lower than allopurinol. 
Antihyperuricemic activity; Invitro study; Invivo study; Stelechocarpus burahol (bl.) Hook. F &; Th.