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8309310 
Meetings & Symposia 
Characterization of activated sludge oil 
Pham, PJ; Hernandez, R; Revellame, ED; Mondala, AH; Cain, JD; French, T; Swalm, DC 
2011 
0 
Abstracts of Papers of the American Chemical Society
ISSN: 0065-7727 
Anaheim, CA 
241st ACS National Meeting and Exposition 
English 
Research on alternative sources of energy is on demand as a result of the current challenges the petroleum industry are facing. The potential of activated sludge for the production of lipid biofuel feedstocks has been demonstrated in our group. In this study, activated sludge oil (ASO) extracted from activated sludges from five Mississippi wastewater treatment facilities was profiled to provide information for activated sludge oil refinement. Activated sludge oil extracted from glucose-supplemented, high C:N ratio wastewater media fermentation samples (Enhanced Activated Sludge) was profiled and compared. Profiles for the ASO hexane (HX) and chloroform (CHCl3) fractions were obtained using gas (GC) with flame ionization and mass spectrometer (MS) detectors. The ASO acetone and methanol fractions were analyzed for glycolipids and phospholipids, respectively. Glycosyl composition was determined through GC and LC-MS. Phospholipid profiles were obtained using 31P NMR Spectroscopy. Methyl palmitate and oleate were the major fatty acid methyl esters present.