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8309450 
Journal Article 
Establishment of ultra performance liquid chromatography (UPLC) protocol for analyzing carotenoids in common wheat 
Li, WS; Xia, XC; He, ZH 
2016 
42 
5 
706-713 
Chinese 
Carotenoids is an important criterion in the assessment of color and nutritional qualities of end-use products in common wheat. In this study, grain powders of Zhongmai 175 (soft wheat) and Zhongyou 206 (hard wheat) were used as samples to develop an optimal procedure for extraction and separation of carotenoids compositions extracted from flours using ultra performance liquid chromatography (UPLC). The most effective extraction of carotenoids extraction was obtained using the solvent system of N-hexane; acetone of 80:20 (v/v, 0.1% BHT w/v) under the oscillation condition of 300 r min-1, 35°C, and 1 h. The separation was conducted using YMC C30 Carotenoid column (100.0 mm × 4.6 mm, 3 μm) with photodiode array (PDA) detector, and the column was thermostated at 35°C. Under a gradient system consisting of acetonitrile:methanol:water:triethylamine (81:14:5:0.05, v/v/v/v) (A) and methanol: ethylacetate:triethylamine (68:32:0.05, v/v/v) (B) at a constant flow rate of 0.4 mL min-1, the carotenoids of common wheat flour samples can be well separated in less than 25 min. Carotenoids were detected at 450 nm. All the present results provided useful information for carotenoids compositions and quality improvement. 
Carotenodis compositions; Triticum aestivum L.; UPLC