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8310119 
Journal Article 
Physico-chemical, chromatographic, and spectrophotometric measurements in the standardization of "Seetharama watee" -a Sri Lankan herbo-mineral formulation 
Hewavithana, T; Ranaweera, KKDS; Tissera, MHA; Yapa, PAJ 
2012 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
4 
SUPPL. 4 
575-578 
English 
"Seetharama watee" is widely used in the febrile illnesses. Although the standardization of this herbo-mineral drug is extremely difficult, it has now become essential for the drug industry. A study was carried to verify whether its physico-chemical fingerprints could be used for its standardization. The minerals in purified forms were mixed with powdered herbs thoroughly and then were ground using different herbal juices oils. Authentically prepared drug was then comparatively assessed with five commercially available counterparts for several parameters related to its physical and chemical properties to evaluate the deviations from the standardized drug. The residue of the ethanol extraction dissolved in ethanol, was used for UV spectrophotometry and HPLC fingerprints. In terms of the acid-insoluble ash, loss on drying, dichloromethane extract and methanol extracts of standard samples were similar to all commercial samples. However, all the samples differed significantly at P < 0.05 level with regard to the weight of a pill, ash content, specific gravity, hexane extract, and ethyl acetate extract values. In the HPLC analysis of the authentically prepared samples, seven major peaks were identified. All samples of "Seetharama watee", had a λ max value between287 - 290 nm. in the spectrophotometric analysis. This study shows that, standardization by assessing the above physical and chemical properties and the HPLC fingerprint profiles and UV spectrophotometric measurements could be considered as a prudent way to achieve a consistent quality with optimal efficacy of "Seetharama watee" authentically prepared. 
High performance liquid chromatography; Seetharama Watee; Standardization; UltraViolet Spectrophotometry; Watika Prakaranaya