Health & Environmental Research Online (HERO)


Print Feedback Export to File
8310196 
Journal Article 
Seclusion of bioactive complexes from Annona muricata leaf extract using TLC 
Rajsekhar, SPV; Helan, CJ; Sridhar, S 
2016 
SPB Pharma Society 
9 
3 
1446-1449 
English 
The current study embraces the extract of Annona muricata leaf using different solvents like chloroform, ethanol and double distilled water. The product yield was calculated and thin layer chromatography (TLC) technique was used to separate the phytocompounds from the crude leaf extract. This technique is being followed from early ages of research and it is a pioneer in the preliminary step in identifying the constituents present in the mixture. The mobile phase used in TLC can be either a combination of two or more solvents depending on the sample extract. The permutation used here were various combination of organic solvent tried. Mobile phase used here were toluene: Ethyl acetate, chloroform: Acetone, chloroform: Ethyl acetate, hexane: Acetone, chloroform: methanol and many more. The best result was analysed in the combination of toluene: Ethyl acetate in ratio of 9:1. Retention Factor (RF) was calculated for each and every combinations of the single compound that travels. 
Annona muricata; Mobile phase; Phytocompounds; RF; TLC