US EPA

8310208 

Journal Article 

Insights on anti-inflammatory effect of sequentially extracted fractions of costus pictus: An in vitro study 

Kalpana, R; Reddy, A 

2016 

Global Research Online 

40 

2 

58-64 

English 

Extracts varying from low to high polarity (Hexane, Dichloromethane (DCM), Ethylacetate (EA) and Methanol) of leaves of of Costus pictus Linn (Family: Costaceae) was assessed for anti-inflammatory activity by in vitro methods. Quantitative analysis of anti-inflammatory components like total amount of phenolics and flavonoids were estimated using spectrophotometric method. In-vitro anti-inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin (Diclofenac) was used as a standard drug for the study of anti-inflammatory activity. Results showed that, the yield was the maximum for the methanolic extract which was about 14.5% and substantially all bioactive phytochemical constituents were at a greater concentration in methanolic extract. It also exhibited the most and significant inhibition of the heat induced albumin denaturation, Red Blood Cells membrane stabilization and proteinase inhibitory effects with 85±1.46, 89±1.24 and 93±0.93% for 250 µg /ml respectively. Total phenol content and flavonoid was the highest for methanolic extract and was estimated as 66.4±1.65 mg of gallic acid equivalents of dry extract and 49.4±1.325 mg of quercitin equivalents per gram of dry extract respectively. The above results help us to substantially conclude that bioactive components are extracted well in high polar solvents which mainly include flavonoids and related polyphenols. These compounds present in C.pictus may be an active lead for potent anti-inflammatory activities. © 2016, Global Research Online. All rights reserved. 

C. pictus; DCM; Diclofenac; EA; Gallic acid; Hexane; Methanol; Quercitin