US EPA

8313492 

Journal Article 

Anti-inflammatory and antinociceptive activities of the hexane extract of lacistema pubescens mart. leaves 

Da Silva, JM; Da Motta, EVS; Mendes, RF; Bellozi, PMQ; De Lima, IVA; Ribeiro, A; Scio, E 

2012 

1 

SPL. 1 

9-15 

English 

Traditionally, leaves of Lacistema genus are used to combat rheumatism, vomiting, dysentery, body aches and as antipyretic Due to the ethnopharmacological importance of this genus, the aims of this study were to investigate the in vivo anti-inflammatory and antinociceptive effects of the hexane extract from the leaves of L pubescens (PHEX) Also, the total amount of flavonoids was determined and the chemical composition of the extract was analyzed The antinociceptive effects were carried out using acetic acid-induced abdominal constriction, formalin and tail flick tests and the anti-inflammatory effect was investigated employing the croton oil induced ear edema in mice The total amount of flavonoids was determined by AlCl 3 reagent method The composition of the extract was analyzed using TLC and GC/MS PHEX significantly decreased the number of acetic acid-induced abdominal contortions being as potent than indomethacin used as reference drug Also PHEX decreased the paw licking time in early (0 - 5 min) and late (15 - 30 min) phases of formalin induced nociception, similar to morphine No effect was observed using tail flick test PHEX showed a topical anti-inflammatory effect on croton oil induced ear edema similar to the anti-inflammatory drug, dexametha sone, but when administered orally, no effect was observed Besides the presence of triterpenes and sterols, phytochemical screening also showed the presence of flavonoids, which constituted 32.7 mg/g of PHEX In conclusion, the findings support some of L pubescens traditional uses involving anti-inflammatory and antino ciceptive effects, and also represent the first report on the chemical profile and biological activities of this specie. 

Anti-inflammatory; Antinociceptive; Lacistema pubescens