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8313729 
Journal Article 
一株多环芳烃降解菌的筛选及其降解特性 
Gao, X; Lin, S; Sun, A; Liao, Y; Zhang, Y; Cai, C 
2020 
Huanjing Gongcheng Xuebao / Chinese Journal of Environmental Engineering
ISSN: 1673-9108 
Science Press 
14 
1668-1678 
Chinese 
Microbial remediation is the main way to remove polycyclic aromatic hydrocarbons (PAHs) from the contaminated soil. It is essential to screen highly efficient degrading bacteria for bioremediation of PAHs-contaminated environment. A PAHs-degrading strain Q3 was isolated from PAHs contaminated soil in Beijing Coking Plant and identified as Rhodococcus rhodochrous by biochemical analysis and 16S rDNA sequence. The results showed the strain Q3 could tolerate pyrene, and could degrade it with initial concentration up to 200 mg•L-1 in liquid medium. Meanwhile, Q3 showed broad-spectra property for PAHs degradation, and could use nine individual PAHs, including phenanthrene, pyrene, benzoanthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, dibenzo[a, h]anthracene or benzo[g, h, i]perylene, as sole carbon source to perform metabolism. Especially for PAHs with high-ring structures, such as benzo[a]pyrene, a relatively high degradation capacity also occurred. In addition, the strain Q3 could effectively degrade the mixed PAHs in the simulated solution, and had a good effect on enhancing remediation for long-term PAHs contaminated soil. The PAHs removal rate in treatment group increased by 24% compared with the control group. The results can provide a technical reference for the bioremediation technology of PAHs contaminated soil. © 2020, Science Press. All right reserved. 
Degradation bacteria; Degradation characteristics; PAHs; Screening; Soil remediation