US EPA

8314169 

Journal Article 

Evaluation of phytochemical characteristics and antimicrobial activity of Pleurotus florida mushroom 

Menaga, D; Mahalingam, PU; Rajakumar, S; Ayyasamy, PM 

2012 

5 

4 

102-106 

English 

Mushrooms are white rot fungi regarded as one of the well known food and possessing various kinds of biopharmaceuticals compounds. Mushrooms are superior to many vegetables based on their nutritional value and it contains 40 - 49% of proteins. The present study consolidates in the aspects of cultivation of Pleurotus florida mushroom with nutrient supplement, biochemical, qualitative and quantitative phytochemical analysis and the antibacterial activity. Pleurotus florida mushroom was cultivated by using Macrotyloma uniflorum (horse gram) as a nutrient supplement substrate; adopted by the "layer spawning" method and the effect of mushroom yield were determined. The fresh fruit bodies of mushroom were harvested, dried, powdered and extracted (by using methanol, ethanol, aqueous, ethylacetate and hexane) using soxhlet apparatus. Then the physico-chemical and antibacterial properties of the extracts were carried out using standard bioassay methods. The horse gram nutrient supplemented batch showed better yield i.e. 23% than control batch. Aqueous, ethanolic and methanolic extracts contains high amount of phenolic compounds, glycosides and flavonoids based on the phytochemical analysis and also strongly inhibited the growth of the both Gram-positive and Gram negative bacterium. Whereas, ethyl acetate and hexane extracts shows low reaction in phytochemical analysis and low antimicrobial activity. Bioactive compounds from Pleurotus florida mushroom extracts could be used as an alternate therapeutics as antibiotics. 

Antibacterial activity; Phytochemicals; Pleurotus florida