US EPA

8314186 

Journal Article 

In vitro antioxidant and anti inflammatory activity of Coccinia grandis 

Ashwini, M; Lather, N; Bole, S; Vedamutrhy, AB; Balu, S 

2012 

1 

International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 

4 

SUPPL.3 

239-242 

English 

Fruit extracts of Coccinia grandis (Cucurbitaceae) are used in India for reducing oxidative stress and inflammation. The objective of this study is to investigate the phyto-chemical constituents, antioxidant and anti-inflammatory activities to justify the use of the plant in folkloric medicine. Phytochemical screening of Coccinia grandis fruit powder in different organic solvents revealed the presence of alkaloids, carbohydrates, phytosterols, resins, tannins, flavonoids, proteins and diterpenes. Antioxidant activities of different fractions from different extracts were evaluated by using InVitro antioxidant assay models like phosphomolybdenum and reducing power assay. Anti-inflammatory activity was evaluated using induced protein denaturation method. The percentage of antioxidant activity was in the order methanol>ethanol>petroleum ether>chloroform>nhexane. Almost all the fractions showed anti-inflammatory activity but n-hexane and petroleum ether showed the highest activity in induced protein denaturation method. The results obtained in this study showed that the fruits of Coccinia grandis have antioxidant and anti-inflammatory properties which provide a basis for the traditional use of the plant. IC50 value was calculated for both antioxidant and anti-inflammatory activity and was found to be 140 μg/ml (methanolic extract) and 100μhg/ml (n-hexane extract) respectively. 

Anti-inflammatory; Antioxidant; Coccinia grandis; Protein denaturation; Reducing power assay